Asexual reproduction may seed new approach for agriculture
Anthony Marrelli
Argus
Farmers throughout the world spend an estimated $36 billion a year to buy seeds for crops, especially those with sought-after traits like hardiness and pest resistance. They can’t grow these seeds themselves because the very act of sexual reproduction erases many of those carefully selected traits. So year after year, farmers must purchase new supplies of specially produced seeds.
This problem is sidestepped by some plants, such as dandelions and poplar trees, which reproduce asexually by essentially cloning themselves. Jean-Philippe Vielle-Calzada, a Howard Hughes Medical Institute (HHMI) international research scholar, wondered whether he could learn enough about the genetics of asexual reproduction to apply it to plants that reproduce sexually.
Vielle-Calzada and his colleagues have developed research that moves us a step closer to turning sexually-reproducing plants into asexual reproducers, a finding that could have profound implications for agriculture. Agricultural companies and farmers around the world have a tremendous interest in this method; it would allow them to simplify the labour-intensive cross-hybridization methods they currently use.
As with animals, sexual reproduction in plants involves the generation of male and female gametes that each carry half of the new organism’s genes. Flowering plants exhibit the most advanced form of sexual plant reproduction, producing pollen derived sperm cells that join with egg cells to produce seeds. Each seed, then, is genetically unique. By contrast, although there are several types of asexual reproduction in plants, they all produce the same result: genetically identical daughter plants.
Vielle-Calzada’s quest to develop an asexual seed began a decade ago, when he decided to investigate apomixis, a specific type of asexual reproduction. Many species of plants use apomixis to generate viable seeds without the fusion of sperm and egg. This method of asexual reproduction results in the formation of seeds that are essentially clones of the main plant and has great potential for crop improvement.
In apomixis, reproductive cells retain the full complement of chromosomes, rather than losing half their genes via meiosis, as happens in sexual reproduction. About 350 families of flowering plants rely on apomixis to reproduce, but nearly all plants used for food reproduce sexually.
Vielle-Calzada and his team studied apomixis in Arabidopsis thaliana, a small flowering mustard plant with a compact, well-understood genome. Arabidopsis was also selected because it reproduces sexually; the team’s goal was to induce apomixis in a species that doesn’t naturally practice it.
The researchers netted a number of interesting genes in their screen, but one in particular, Argonaute 9, caught their attention immediately. The large family of Argonaute proteins has gained widespread attention among researchers because the proteins control which gene products (either RNA or proteins) a cell makes. Argonautes do this by slicing up messenger RNA before it can be translated into proteins.
The next step involved mutating the Argonaute 9 gene to produce several gametes, rather than the usual single gamete. Instead of carrying half of the species’ chromosomes, they carried the full complement of genetic material, implying that they had not undergone meiosis.
By cutting off the function of Argonaute, the scientists caused a schizophrenic reaction of the cells in the ovule, which were not supposed to become gametes. It seems that Argonaute normally prevents those cells from being transformed into gamete precursors.
This suggests that Argonaute 9 prevents the initiation of apomixis in Arabidopsis.
The finding raises the possibility that many, or maybe even all, plants have the ability to reproduce through apomixis, but that potential is suppressed by Argonaute 9. It’s possible that plants have an ancient genetic memory that allows them to reproduce asexually.
The team then searched inside the ovule to look for the pieces of RNA that Argonaute 9 degraded. They found that Argonaute chewed up 2,600 snippets of RNA. After mapping those RNA sequences back to the Arabidopsis genome, the team discovered that more than half were produced by transposons. Transposons, also called “jumping genes”, are mobile genetic elements that copy and insert themselves throughout the genome. Their function remains somewhat mysterious, although some evidence suggest they are important in controlling gene expression.
“It seems that Argonaute 9 silences transposons in the ovule of Arabidopsis”, Vielle-Calzada says. The open question now is why this occurs. His working hypothesis is that squelching the transposons prevents apomixis, but his lab is working to prove the connection.
Though he has made great progress, Vielle-Calzada is still working toward creating a fully asexual Arabidopsis plant. His current mutants do not develop completely asexual seeds. But by highlighting the role of Argonaute 9 in plant reproduction, Vielle-Calzada has moved a step closer to a slew of agricultural possibilities.